IIT JAM biotechnology syllabus is divided into four parts: biology, chemistry, mathematics, and physics.

Biology and chemistry courses are based on graduate-level courses, and math and physics courses are based on 10 + 2 level courses.

Applicants can check the detailed IIT JAM syllabus for Biotechnology 2022 from here as well as the official website of IIT JAM.


Cell Biology

Structure of prokaryotic and eukaryotic cells

Membrane structure and function

Organelles and internal organization of the eukaryotic cell Protein trafficking in a eukaryotic cell

Cell communication - signalling pathways: endocrine and paracrine signalling

Extracellular matrix and apoptosis

Cell cycle - stages of mitosis and meiosis, and control of cell division cycle


Structure and function of biological macromolecules


Enzymes - basic mechanisms of enzyme catalysis, Michaelis-Menten kinetics, enzyme inhibition, vitamins as coenzymes, and regulation

Bioenergetics - free-energy change, high-energy compounds, biological oxidation-reduction reactions and reduction potential

Metabolism - glycolysis, TCA cycle, oxidative phosphorylation, photosynthesis, nitrogen fixation, urea cycle, and regulation of glycolysis and TCA cycle.


Mendel's laws

Inheritance patterns of polygenic traits

Mendelian inheritance patterns of human disorders

Pedigree analysis

Chromosomal basis of inheritance

Genetic recombination

Mapping genes on chromosomes based on linkage analysis

Plant breeding.

Molecular Biology

Landmark experiments that established DNA is the genetic material

DNA replication

Proofreading and repair of DNA

DNA recombination


RNA processing


Regulation of gene expression including operons bacteria and differential gene expression in multicellular eukaryotes.


Darwinian view - natural selection, fossil record and descent with modification

Population genetics - sources of genetic variation, gene pools and allele frequencies, HardyWeinberg equation, genetic drift, gene flow and adaptive evolution

Different types of speciation

Phylogenetic classification

Origin of life - abiotic synthesis of biological macromolecules, protocell, dating fossils and origin of multicellularity.




Structural features of viruses, bacteria, fungi and protozoa



Nutrition-based classification of microbes

Microbial metabolism

Growth kinetics

Submerged fermentation techniques

Microbial genetics.

Plant Biology

Types of tissues and organs

Primary and secondary growth


ransport in vascular plants

Plant nutrition

Development of flowering plants - gametophytic and sporophytic generations, different developmental phases, genetic control of flowering, gametogenesis, incompatibility, embryogenesis, dormancy, germination and environmental influence

Plant hormones


Plant response to biotic and abiotic stresses

Animal Biology

Digestive, circulatory, respiratory, excretory, nervous, reproductive and endocrine systems

Basics of immunology - Innate and adaptive immunity, Immune cells and Immunoglobulins

Animal development - Fertilization, embryonic pattern formation, cleavage, gastrulation, cellular differentiation and morphogenesis.


Climate patterns

Terrestrial and aquatic biomes

Environmental constraints on species distribution

Factors affecting population density

Interactions among communities


Ecological remediation.

Methods in Biology

Cell Biology

Microscopy (light microscopy and electron microscopy)

Staining proteins with antibodies

Visualizations using the GFP reporter.

Biochemical techniques

UV spectrophotometry

Biomolecular chromatography

cell fractionation by centrifugation


Western blotting.

Molecular biology techniques

DNA cloning - plasmid vectors, and restriction enzymes

Polymerase Chain


Expression of cloned eukaryotic genes in bacteria

Hybridization techniques

DNA sequencing.


Plant tissue culture

Cloning of animals through somatic cell nuclear transfer

Applications of recombinant DNA technology in medicine, agriculture and forensic science.


Structure and properties of Atoms

Bohr's theory

Periodicity in properties.

Bonding in molecules

Chemical bonding

Complex formation

Physical and chemical basis of molecular interactions.

Chemical kinetics, thermodynamics, and equilibrium

Chemical equilibrium

Chemical thermodynamics (first and second law)

Chemical kinetics (zero and first order reactions).

Instrumental techniques - Spectroscopy

fundamentals of molecular spectroscopy, emission

absorption spectroscopy, UV-Vis, IR and 1-D proton NMR spectroscopy, basics of mass spectrometry

Basics of calorimetry

Basic concepts of crystallography.

Physical and chemical properties of compounds

Chemical catalysis

Acid-base concepts

Concepts of pH and buffer

Conjugative effects and resonance

Inductive effects

Electromeric effects



Chemistry of organic compounds


Alkyl halides




Carboxylic acids

Amines and their derivatives

Aromatic hydrocarbons, halides, nitro and amino compounds, phenols, diazonium salts, carboxylic and sulphonic acids

soaps and detergents

Stereochemistry of carbon compounds.



Relations and Functions

Mathematical Induction

Logarithms; Complex numbers

Linear and Quadratic equations

Sequences and Series


Cartesian System of Rectangular Coordinates;

Straight lines and Family

Three Dimensional Geometry

Permutations and Combinations

Binomial Theorem


Matrices and Determinants

Boolean Algebra


Limits and Continuity;


Ordinary Differential Equations

Application of Derivatives

Integration as inverse process of differentiation

Definite and indefinite integrals

Methods of Integration

Integration by parts.


Measures of dispersion

Mean Deviation for grouped and ungrouped data

Variance and Standard Deviation

and Analysis of Frequency Distribution.


Random Experiments


Axiomatic Approach to Probability

Conditional Probability and its properties

Multiplication Theorem on Probability

Independent Events

Bayes' Theorem

Random Variables and its Probability Distributions

Bernoulli Trails and Binomial Distributions.


Units and measurements

Motion in one and two dimensions

Laws of motion

Work and kinetic energy

Conservation of energy

System of particles and rotational motion

Mechanical properties of solids and fluids

Thermal properties of matter

Heat and laws of thermodynamics

Kinetic theory of gases

Electric charge and field

Electric potential and capacitance

Current, resistance and simple circuits

Moving charges and magnetic field

Magnetism and matter

Electromagnetic induction

Electromagnetic waves

Alternating currents

Optics: Geometrical Optics - Reflection by spherical mirrors, Refraction at spherical surfaces

lenses, Total internal reflection and Optical instruments

Wave optics - Reflection and refraction of plane waves, Interference, Diffraction, Polarization,

Young's experiment: Dual nature of radiation and matter

Atoms, nuclei and nuclear physics

Semiconductor materials, devices and simple circuits.