| S. No |
PART-B SYLLABUS |
| 1 |
Biomolecules-structure and functions; Biological membranes, structure, action potential
and transport processes; Enzymes- classification, kinetics and mechanism of action; Basic
concepts and designs of metabolism (carbohydrates, lipids, amino acids and nucleic acids)
photosynthesis, respiration and electron transport chain; Bioenergetics. |
| 2 |
Viruses- structure and classification; Microbial classification and diversity(bacterial, algal and
fungal); Methods in microbiology; Microbial growth and nutrition; Aerobic and
anaerobic respiration; Nitrogen fixation; Microbial diseases and host-pathogen interaction.
Prokaryotic and eukaryotic cell structure; Cell cycle and cell growth control; Cell-Cell
communication, Cell signaling and signal transduction. |
| 3 |
Molecular structure of genes and chromosomes; Mutations and mutagenesis; Nucleic acid
replication, transcription, translation and their regulatory mechanisms in prokaryotes and
eukaryotes; Mendelian inheritance; Gene interaction; Complementation; Linkage,
recombination and chromosome mapping; Extra chromosomal inheritance; Microbial
genetics (plasmids, transformation, transduction, conjugation); Horizontal gene transfer and
Transposable elements; RNA interference; DNA damage and repair; Chromosomal variation;
Molecular basis of genetic diseases. |
| 4 |
genetics (plasmids, transformation, transduction, conjugation); Horizontal gene transfer and
Transposable elements; RNA interference; DNA damage and repair; Chromosomal variation;
Molecular basis of genetic diseases.
Principles of microscopy-light, electron, fluorescent and confocal; Centrifugation- high
speed and ultra; Principles of spectroscopy-UV, visible, CD, IR, FTIR, Raman, MS,NMR;
Principles of chromatography- ion exchange, gel filtration, hydrophobic interaction, affinity,
GC,HPLC, FPLC; Electrophoresis; Microarray. |
| 5 |
History of Immunology; Innate, humoral and cell mediated immunity; Antigen; Antibody
structure and function; Molecular basis of antibody diversity; Synthesis of antibody and
secretion; Antigen-antibody reaction; Complement; Primary and secondary lymphoid organ;
B and T cells and macrophages; Major histocompatibility complex (MHC); Antigen
processing and presentation; Polyclonal and monoclonal antibody; Regulation of immune
response; Immune tolerance; Hypersensitivity; Autoimmunity; Graft versus host reaction. |
| 6 |
Major bioinformatics resources and search tools; Sequence and structure databases;
Sequence analysis (biomolecular sequence file formats, scoring matrices, sequence
alignment, phylogeny); Data mining and analytical tools for genomic and proteomic studies;
Molecular dynamics and simulations (basic concepts including force fields, protein-protein,
protein-nucleic acid, protein- ligand interaction). |
| 7 |
Restriction and modification enzymes; Vectors; plasmid, bacteriophage and other viral
vectors, cosmids, Ti plasmid, yeast artificial chromosome; mammalian and plant expression
vectors; cDNA and genomic DNA library; Gene isolation, cloning and expression ;
Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain
reactions; DNA fingerprinting; Southern and northern blotting; In- situ hybridization; RAPD,
RFLP; Site-directed mutagenesis; Gene transfer technologies; Gene therapy. |
| 8 |
Totipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture
and Cell suspension culture system: methodology, kinetics of growth and, nutrient
optimization; Production of secondary metabolites by plant suspension cultures; Hairy root
culture; transgenic plants; Plant products of industrial importance. |
| 9 |
Animal cell culture; media composition and growth conditions; Animal cell and tissue
preservation; Anchorage and non-anchorage dependent cell culture; Kinetics of cell
growth; Micro & macro-carrier culture; Hybridoma technology; Stem cell technology; Animal
cloning; Transgenic animals. |
| 10 |
Chemical engineering principles applied to biological system, Principle of reactor design,
ideal and non- ideal multiphase bioreactors, mass and heat transfer; Rheology of
fermentation fluids, Aeration and agitation; Media formulation and optimization; Kinetics of
microbial growth, substrate utilization and product formation; Sterilization of air and media;
Batch, fed-batch and continuous processes; Various types of microbial and enzyme
reactors; Instrumentation control and optimization; Unit operations in solid-liquid separation
and liquid-liquid extraction; Process scale-up, economics and feasibility analysis. |
| 11 |
Engineering principle of bioprocessing - Upstream production and downstream; Bioprocess
design and development from lab to industrial scale; Microbial, animal and plant cell culture
platforms; Production of biomass and primary/secondary metabolites; Biofuels, Bioplastics,
industrial enzymes, antibiotics; Large scale production and purification of recombinant
proteins; Industrial application of chromatographic and membrane based bio-separation
methods; Immobilization of biocatalysts (enzymes and cells) for bioconversion processes;
Bioremediation-Aerobic and anaerobic processes for stabilization of solid / liquid wastes. |
| 12 |
Tissue culture and its application, Micropropagation. Meristem culture and production
of virus-free plants. Anther and microspore culture. Embryo and ovary culture.
Protoplast isolation. Protoplast fusion-somatic hybrids, cybrids. Somaclones.
Synthetic seeds. In vitro germplasm conservation. Cryopreservation. Organelle DNA,
Satellite-and repetitive DNAs. DNA repair. Regulation of gene expression.
Recombinant DNA technology-cloning vectors, restriction enzymes, gene cloning.
Methods of gene transfer in plants. Achievements and recent developments of genetic
engineering in agriculture. Development of transgenics for biotic & abiotic stress
tolerance, bioethics, terminator technology, nanotechnology, DNA fingerprinting, gene silencing. |
