GAT-B SYLLABUS


GAT - B Syllabus designed for to crack the examination & obtain better rank in GAT - B entrance Exam, Comprises 2 sections PART A and PART B. Candidates by now can check the subject wise Detailed GAT - B syllabus

PART- A

Questions of the level of 10+2 in the subjects: Physics, Chemistry, Mathematics and Biology.

PART- B

Biomolecules-structure and functions

Biological membranes, structure, action potential and transport processes

Enzymes- classification, kinetics and mechanism of action

Basic concepts and designs of metabolism (carbohydrates, lipids, amino acids and nucleic acids) photosynthesis, respiration and electron transport chain

Bioenergetics.

Viruses- structure and classification

Microbial classification and diversity(bacterial, algal and fungal)

Methods in microbiology

Microbial growth and nutrition

Aerobic and anaerobic respiration

Nitrogen fixation; Microbial diseases and host-pathogen interaction.

Prokaryotic and eukaryotic cell structure

Cell cycle and cell growth control

Cell-Cell communication, Cell signaling and signal transduction.

Molecular structure of genes and chromosomes

Mutations and mutagenesis

Nucleic acid replication, transcription, translation and their regulatory mechanisms in prokaryotes and eukaryotes

Mendelian inheritance

Gene interaction

Complementation

Linkage, recombination and chromosome mapping

Extra chromosomal inheritance

Microbial genetics (plasmids, transformation, transduction, conjugation)

Horizontal gene transfer and Transposable elements

RNA interference

DNA damage and repair

Chromosomal variation

Molecular basis of genetic diseases.

Principles of microscopy-light, electron, fluorescent and confocal

Centrifugation- high speed and ultra

Principles of spectroscopy-UV, visible, CD, IR, FTIR, Raman, MS,NMR

Principles of chromatography- ion exchange, gel filtration, hydrophobic interaction, affinity, GC,HPLC, FPLC

Electrophoresis

Microarray.

History of Immunology

Innate, humoral and cell mediated immunity

Antigen

Antibody structure and function

Molecular basis of antibody diversity

Synthesis of antibody and secretion

Antigen-antibody reaction; Complement

Primary and secondary lymphoid organ

B and T cells and macrophages

Major histocompatibility complex (MHC)

Antigen processing and presentation

Polyclonal and monoclonal antibody

Regulation of immune response

Immune tolerance

Hypersensitivity

Autoimmunity

Graft versus host reaction.

Major bioinformatics resources and search tools

Sequence and structure databases

Sequence analysis (biomolecular sequence file formats, scoring matrices, sequence alignment, phylogeny)

Data mining and analytical tools for genomic and proteomic studies

Molecular dynamics and simulations (basic concepts including force fields, protein-protein, protein-nucleic acid, protein- ligand interaction)

Restriction and modification enzymes

Vectors

plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome

mammalian and plant expression vectors

cDNA and genomic DNA library

Gene isolation, cloning and expression

Transposons and gene targeting

DNA labeling

DNA sequencing

Polymerase chain reactions

DNA fingerprinting

Southern and northern blotting

In- situ hybridization

RAPD, RFLP

Site-directed mutagenesis

Gene transfer technologies

Gene therapy.

Totipotency

Regeneration of plants

Plant growth regulators and elicitors

Tissue culture and Cell suspension culture system: methodology, kinetics of growth and, nutrient optimization

Production of secondary metabolites by plant suspension cultures

Hairy root culture

transgenic plants

Plant products of industrial importance.

Animal cell culture

media composition and growth conditions

Animal cell and tissue preservation

Anchorage and non-anchorage dependent cell culture

Kinetics of cell growth

Micro & macro-carrier culture

Hybridoma technology

Stem cell technology

Animal cloning

Transgenic animals.

Chemical engineering principles applied to biological system, Principle of reactor design, ideal and non- ideal multiphase bioreactors, mass and heat transfer

Rheology of fermentation fluids, Aeration and agitation

Media formulation and optimization

Kinetics of microbial growth, substrate utilization and product formation

Sterilization of air and media

Batch, fed-batch and continuous processes

Various types of microbial and enzyme reactors

Instrumentation control and optimization

Unit operations in solid-liquid separation and liquid-liquid extraction

Process scale-up, economics and feasibility analysis.

Engineering principle of bioprocessing - Upstream production and downstream

Bioprocess design and development from lab to industrial scale

Microbial, animal and plant cell culture platforms

Production of biomass and primary/secondary metabolites

Biofuels, Bioplastics, industrial enzymes, antibiotics

Large scale production and purification of recombinant proteins

Industrial application of chromatographic and membrane based bio-separation methods

Immobilization of biocatalysts (enzymes and cells) for bioconversion processes

Bioremediation-Aerobic and anaerobic processes for stabilization of solid / liquid wastes.

Tissue culture and its application, Micropropagation

Meristem culture and production of virus-free plants

Anther and microspore culture

Embryo and ovary culture

Protoplast isolation

Protoplast fusion-somatic hybrids, cybrids

Somaclones

Synthetic seeds

In vitro germplasm conservation

Cryopreservation

Organelle DNA, Satellite-and repetitive DNAs

DNA repair

Regulation of gene expression

Recombinant DNA technology-cloning vectors, restriction enzymes, gene cloning

Methods of gene transfer in plants

Achievements and recent developments of genetic engineering in agriculture

Development of transgenics for biotic & abiotic stress tolerance, bioethics, terminator technology, nanotechnology, DNA fingerprinting, gene silencing.